This antibody targets a specific epitope within the internal region of synaptopodin/SYNPO, a proline-rich actin-binding protein featuring two actin-binding sites. Synaptopodin, an actin-binding protein, was initially identified in podocytes and a subset of telencephalic postsynaptic densities. In human tissue, synaptopodin has a molecular weight of 73.7 kD and pI of 9.38, while in mouse, it's 74 kD and pI 9.27. In SDS-PAGE, the antigen manifests as a 100 kD polypeptide in brain and 110 kD in kidney, attributed to post-translational modifications. In Western blot analysis, the antibody also interacts with a 44 kD degradation fragment of synaptopodin.