The QuantiGene Plex Rat Housekeeping Gene Panel, 10-plex, facilitates the measurement of key rat housekeeping gene expressions by analyzing 10 RNA targets and biomarkers in a single well, utilizing Luminex xMAP technology and branched DNA signal amplification. This panel comprises commonly used reference genes for reliable normalization and quality control in gene expression studies. QuantiGene Plex assays, hybridization-based, enable direct RNA quantitation from cell lysates, tissue homogenates, or purified RNA, allowing for multiplexing of multiple targets in the same well. The panel includes target-specific probes and magnetic capture beads, with fluorescent signals amplified and read using a Luminex xMAP instrument. Signal intensity correlates with target mRNA levels, enabling (relative) gene expression quantification and human immune profiling. The QuantiGene Plex data analysis app offers detailed, step-by-step data analysis. Research applications include compound screening, biomarker verification, siRNA efficiency determination, clinical sample analysis, microarray validation, predictive toxicology research, and translocation/fusion gene detection, making it suitable for any multiplex gene expression analysis, regardless of species or sample type.