Ascentis Express HPLC columns, through the use of Fused-Core particle technology, can provide you with both the high speed and high efficiencies of sub-2 µm particles while maintaining lower backpressures. The combination of high efficiency and low backpressure benefits UPLC (or other ultra high pressure system) users, as well as conventional HPLC users.. Parameter: 60 °C temp. range. Application(s): HPLC: suitable. Sold in each. . . Sigma-Aldrich 53822-U Ascentis Express C18 HPLC Column, 2.7 µm Particle Size, 2.1mm ID, 5cm Length. Country of Origin : United States. Part Number : 1188M67EA. Pack of : 1.