Limited lifetime: typically tens to hundreds of feeds.
High separation efficiency: suitable for high resolution separation of complex mixtures.
Reusable: can be cleaned and regenerated under appropriate conditions for multiple analyses.
Strict conditions of use: sensitive to pH, temperature, flow rate, etc., need to be precisely controlled to extend the life of the column.
Fine packing structure of liquid chromatography columns: the columns are filled with micron-sized particles (commonly 3-5 µm), which have a high specific surface area and improve the separation efficiency.
