Miniature affinity purification using antibody-bound solid support
Protein of interest securely attached to resin in favorable binding conditions
Non-target components rinsed off post-binding
Target molecule freed and retrieved from ligand
وصف
Affinity purification typically includes these steps: Mix a crude sample (like cell lysate, culture supernatant, or serum) with an affinity support, allowing the target molecule to bind to the immobilized ligand. Wash away unbound sample components using suitable buffers that preserve the binding interaction. Elute the target molecule from the ligand by changing buffer conditions to disrupt the binding.