ACTaq Blue / Red Master mix is complete "ready-to-go" 2X reaction mix which only requires consumer researcher to add water, template and primers. ACTaq Blue / Red Master mix differs from ACTaq Master mix such that it contains blue / red dye added to the enzyme used for tracking and loading dye purposes, and therefore no loading buffer is required. Taq ACTaq DNA Polymerase originates from Thermus Aquatics with molecular weight of 94KDa. For use in DNA fragment extension amplification reactions with DNA having dA overhang on 3' ends; at extension amplification speed of 1200 bases per minute and optimized between 65-75°C. Optimized for best performance when using dNTP concentration at 100-300mM, 1.5-3.0mM Mg2+ concentration, and pH at 8.1-9.2.