For rapid, quantitative determination of monoamine oxidase activity and MAO inhibitor screen.
Method: Fluorometric assay (530/585nm). A spectrophotometer or plate reader is required, but not provided.
Samples: MAO enzyme preparations. Species: All. Detects 0.01 U/L
Procedure: 30 min. Size: 100 tests per kit
For Research Use Only. Not for Diagnostic Purposes
Description
Monoamine oxidases (MAO, EC 1.4.3.4) are a family of mitochondrial enzymes that catalyze the oxidative deamination of monoamines. BioAssay Systems' MAO Assay Kit provides a convenient fluorimetric means to measure MAO enzyme activity. In the assay, MAO reacts with p-tyramine, a substrate for both MAO-A and MAO-B, resulting in the formation of H2O2, which is determined by a fluorimetric method (em/ex = 585/530nm). The assay is simple, sensitive, stable and high-throughput adaptable. This product is for Research Use only and is not intended for diagnostic purposes.