Teach your students about restriction enzyme digests in the context of forensic science!
Your students will cut DNA with restriction enzymes and then compare the banding pattern of the crime scene DNA versus that of two suspects using agarose gel electrophoresis.
Group Size:
For 8 gels
Time Required:
Complete in 1 hour 20 minutes to 1 hour 45 minutes
Kit Includes:
Instructions, “crime scene” and "suspect" Ready-to-Load DNA samples, DNA Standard Marker, Dryzymes - Eco RI and Hind III, Enzyme Reaction Buffer, Reconstitution Buffer, UltraSpec-Agarose, 10X Gel Loading Solution, 50X Concentrated Electrophoresis Buffer, Practice Gel Loading Solution, SYBR Safe stain, FlashBlue DNA Stain, 1 mL pipet, Microtipped Transfer Pipets, & Microcentrifuge Tubes.
All You Need:
DNA Electrophoresis, Micropipettes: 5-50 µl, Water Bath, Blue Light Visualization System (use if staining with SYBR Safe) White Light Box (use if staining with FlashBlue), & Microwave or Hot plate.
Storage:
Some Components Require Freezer Storage Upon Receipt