Radiantª Extract & Amplify Tissue PCR Kit combines rapid DNA extraction with fast, highly specific DNA amplification in a convenient, easy to use format. Eliminate the need for overnight & time intensive DNA extraction methods with this simple, integrated extraction and amplification PCR kit powered by the latest advances in hot-start polymerase technology. Radiantª Extract & Amplify Tissue PCR Kit has been developed for fast, efficient amplification of DNA from a variety of tissues and is particularly suited to solid tissue such as mouse tail or mouse ear. DNA extraction is performed in a single tube, removing the need for multiple washing steps. Extraction of DNA is rapid, providing DNA for PCR in 15 minutes. Extraction takes place in a single tube, minimizing potential contamination. Extracted DNA is amplified using supplied 2X Radiantª RED Hot-Start Taq Mix. Our antibody hot start polymerase uses the latest developments in polymerase technology and buffer chemistry to enhance PCR speed, yield and sensitivity. The completed reaction is ready for direct gel loading without the need to add loading buffer. Per 80 Reactions, (2 x 1mL 2X HS Taq Red, 1 x 1.6mL 5X Extract Buffer A, 1 x .08mL 10X Extract Buffer B). Per 400 Reactions, (10 x 1mL 2X HS Taq Red, 5 x 1.6mL 5X Extract Buffer A, 5 x .08mL 10X Extract Buffer B) 15 minute extraction process NO OVERNIGHT lysing/extracting PCR with supplied mix RED loading buffer included in PCR mix