💖Usage method Usage method of the glass grinder: After washing thoroughly, dry it. If it's too dirty, soak it in acid before washing. 2. Select the appropriate homogenizing buffer and prepare it properly. 3. Homogenize on ice, and the number of homogenations should be in accordance with the experimental sample It depends on the quality and the purpose. After homogenization is completed, pour out the sample. 5. After cleaning as per step 1, dry and store.
💖Quick questions and quick answers: Question: Must animal tissues be cut into very small pieces before grinding them with a glass tissue homogenizer? Answer: This is very necessary because it will be easier to grind 2. Q: When using a glass tissue homogenizer, is it necessary to use homogenization buffer solution? Answer: It is generally recommended to use homogenizing buffer 3. Q: What is the function of the homogenizing buffer solution? Answer: On the one hand, it is easy to pour out; on the other hand, it is to stabilize certain substances after cell disruption or facilitate separation. If Ca2+ is added to the buffer solution, calcium ions can promote the aggregation of plasma membrane fragments. As for what to use, it depends on what kind of tissue is being ground. 4. Q: How to choose what kind of homogenizing buffer to use? Answer: The most commonly used homogenizing media are isotonic sucrose (0.25mol/L) and potassium chloride (0.154mol/L). Sucrose is the preferred choice for the research on the separation of classic subcellular components, while potassium chloride is more suitable for the study of exogenous chemical metabolic enzymes. For instance, it can more effectively remove hemoglobin from microsomal preparations and reduce the interference in spectral determination. The pH of the homogenizing medium should be adjusted to around 7.4 before use. The homogenizing buffer may contain 5 to 50mmol/L of Tris or Hepes. The commonly used homogenization buffer in most studies is a 50mmol/LTris·HCI buffer containing 0.154mol/ L KCL, with a pH of 7.4. This buffer solution can be stored for at least 12 weeks without deterioration at 4℃.