For use with hybridization incubators with rotators, the tubes offer a cleaner and safer alternative to hybridizing in bags. Multiple Northern, Southern or Western blots (up to 5 per tube) can be hybridized in a single tube, using nylon mesh sheets to separate the hybridization membranes. Probe volumes are significantly reduced with tubes; approximately 5 mL of probe per blot. Use one long (300 mm) tube per rotator position or two short (150 mm) tubes per position (one tube per clamp) in most standard hybridization incubators. Tubes are complete with 45 mm polypropylene caps and silicone o-rings.