The SIHA NF-kB-RFP reporter stable cell line is an engineered human cervical carcinoma cell line featuring a stable reporter system for dynamic, non-invasive cellular and molecular analysis. This dual combination of a disease-relevant cancer model and a reporter readout offers a potent tool for both fundamental and translational cancer research. Designed for flexible multiplexing, it enables dual-reporter assays in co-culture or multi-pathway studies, ensuring consistent results with stable and reproducible reporter expression. Derived from a primary cervical squamous cell carcinoma, it contains 1-2 copies of integrated HPV-16 DNA, making it ideal for studying HPV E6-mediated degradation. The NF-kB pathway activation responsive promoter facilitates effective transcriptional regulation and signaling analysis.