The DMEM/F12 medium, an enhanced version of DMEM with additional nutrients from F12, is rich in trace elements and widely employed in cultivating various mammalian cells. It serves as a foundation for developing serum-free media and is suitable for mammalian cell culture and clone density culture under low serum conditions. This product comprises diverse amino acids, vitamins, and inorganic salts for cell culture, excluding proteins, lipids, and growth factors. Thus, it should be used with serum or serum-free additives.