This Rat Aortic Smooth Muscle Cells Isolation and Culture Kit is specifically designed for extracting primary rat Aortic Smooth Muscle Cells. Standard use of the kit allows for the acquisition of one flask of cells (T-25 culture flask) per test, with a cell count exceeding 1×10⁶ cells. When passaged at a 1:2 ratio, the cells can undergo 5 passages, with the best cell state within the first 3 passages. Immunofluorescence analysis confirms that the cell purity (α-SMA-positive rate) exceeds 90%.