YPD is a nutritionally rich medium for the growth of S. cerevisiae, containing dextrose, peptone and yeast extract. Media Preparation: Reagents are tested and chosen for their ability to promote optimum growth of S. cerevisiae when combined in YPD Broth formulations. Chosen reagents are combined in the proper proportions according to the YPD formulation and thoroughly blended for a uniform distribution. A sample of the blended formulation is used to prepare agar medium. The combinations are mixed to dissolve components and then autoclaved at 121°C for 15 minutes.