Non-specific Interactions: Primers may bind incorrectly
Contaminated Template: cDNA may be compromised
Improper Dilution: Preamplification product not diluted correctly
High Threshold: Low miRNA expression may not be detected
Uneven Reconstitution: Dried-down assays may cause baseline dip
Card Sealing Issues: Empty wells or leaks due to improper sealing
Improper Mix Preparation: PCR reaction mix may be flawed
وصف
Megaplex RT Primers are designed for use with TaqMan MicroRNA assays, serving as primers for reverse transcription to amplify the template before real-time PCR. They are designed to be used with the Megaplex RT Primers for Rodent Pool A, a set of primers for microRNA analysis.