Boost RNA input in reverse transcription, if feasible
Increase cDNA volume in qPCR reaction (up to 20%)
Attempt a different reverse transcription kit, like SuperScript VILO Master Mix, for optimal cDNA yield
Consider one-step or Cells-to-CT workflow based on your sample type
وصف
The Power SYBR Green RNA-to-CT 1-Step Kit is a streamlined real-time PCR kit, offering efficient and sensitive gene expression detection. It employs a unique hydrophobic dye to expedite RNA-to-Ct conversion, ensuring swift and dependable amplification. The kit includes a user-provided specific primer set targeting the gene of interest, guaranteeing specificity and sensitivity. It's designed for use with TaqMan assays and can be readily adapted for other real-time PCR applications.
