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بيت / Thermo Fisher Scientific / لوازم علوم الحياة
التقاط RNA/DNA محدد بواسطة Thermo Fisher Scientific in Kuwait

التقاط RNA/DNA محدد بواسطة Thermo Fisher Scientific

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مميزات خاصة

  • Extracting RNA/DNA pathogens
  • Subtractive hybridization process
  • cDNA enrichment and selection
  • Identifying and isolating mutated sequences
  • Isolating cell-specific transcripts
  • mRNA differential display analysis

وصف

The direct capture method involves immobilizing double-stranded PCR products onto beads, which can then be easily converted into single-stranded bead-bound templates for capturing specific RNA or DNA molecules directly from solution. Alternatively, an indirect capture approach can offer faster reaction kinetics in certain cases. This indirect method allows the target sequence to be captured before being immobilized onto magnetic beads. First, a biotinylated capture-sequence (single-stranded DNA) is incubated with the sample and allowed to hybridize with the targeted RNA or DNA molecules in solution. Streptavidin-coated Dynabeads are then added to the mixture, and the hybridized sequences are immobilized onto the Dynabeads via the streptavidin-biotin bond.

منتجات شبيهه


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